Newborn Cells in the Epileptic Brain Provide a Potential Target for Anti-Seizure Medications

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Altered Cells Create an Electrical “Fire” in patients With Epilepsy

Altered cells create an electrical “fire” in patients with epilepsy. Credit: BioRender illustration by Aswathy Ammothumkandy/Bonaguidi Lab/USC Stem Cell

Over the years, everyone loses a few brain cells. A study led by scientists from

Newborn Astroglia in Brain Tissue

Newborn astroglia (green) in brain tissue from human epilepsy patients. Credit: Image by Aswathy Ammothumkandy/Bonaguidi Lab/USC Stem Cell

The surgical specimens afforded a unique opportunity for the researchers to study living brain tissue from patients with epilepsy, and to compare its microscopic anatomy with post-mortem samples from people with no known neurological disease.

In the samples from people both with and without epilepsy, the scientists observed newborn neurons, adding compelling new evidence to the ongoing scientific debate about whether adults retain the ability to generate these cells. In the surgical specimens, the longer the patients had experienced seizures, the scarcer these newborn neurons became. More surprisingly, the surgical specimens contained a persistent population of immature astroglia that were not observed in the disease-free samples.

Because the brain tissue in the surgical specimens was still alive, the scientists could also use it to grow stem cells in the laboratory and test their ability to form newborn neurons and immature astrocytes. In these experiments, a longer disease duration reduced the ability to form newborn neurons and increased the production of immature astroglia, consistent with the team’s direct observations of the surgical specimens.

The team also studied electrical activity related to seizures. They found suspicious correlations between where electrical activity was localized within the surgical samples, and the location and behavior of the astroglia.

“Normally, astroglia are considered to be supporting cells, because their job is to create an environment where neurons can thrive,” said Ammothumkandy. “But in patients who have lived for many years with epilepsy, it might be immature astroglia that are contributing to both initiating and modulating chronic seizures.”

If this is the case, then immature astroglia could be an effective cell type to target by developing an entirely new class of anti-seizure medications.

“Currently available seizure medications tend to target neurons, so medications that act on immature astroglia could greatly expand the options for our patients,” said Liu, a professor of neurological surgery, neurology, and biomedical engineering, director of the USC Neurorestoration Center, and director of the USC Epilepsy Care Consortium. “A new class of drugs could combine with current medical and surgical strategies to control seizures without aggressive surgical removal of parts of the brain that can be critically important for learning, memory and emotional regulation.”

Reference: “Altered adult neurogenesis and gliogenesis in patients with mesial temporal lobe epilepsy” 5 April 2022, Nature Neuroscience.


DOI: 10.1038/s41593-022-01044-2

Bonaguidi, Liu, and Russin originally kicked off the project with pilot funding from an Eli and Edythe Broad Innovation Award, which supports faculty pursuing research collaborations related to stem cells. The study brought together clinicians, scientists and engineers from across the Keck School of Medicine of USC—including at the Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, the USC Neurorestoration Center, and the Zilkha Neurogenetic—the USC Epilepsy Care Consortium, the USC Viterbi School of Engineering, and the USC Davis School of Gerontology, as well as other universities and medical centers.

About the study

Additional co-authors include: Kristine Ravina, Victoria Wolseley, Pen-Ning Yu, Luis Corona, Naibo Zhang, George Nune, Laura Kalayjian, Brian Lee, Dong Song, Theodore W. Berger, Christianne Heck, and Robert H. Chow from USC; Jason A. D. Smith from USC and the University of Texas Southwestern Medical Center; Alexandria N. Tartt from the New York State Psychiatric Institute; J. John Mann, Victoria Arango, and Maura Boldrini from the New York State Psychiatric Institute and Read More

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